Method and kit for detecting stem cell

A method for detecting a stem cell by detecting a sugar chain represented by (Formula 1) below:

• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule,
• or (Formula 2) below:
• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule, contained in a culture supernatant or a lysate of cells,
• the method comprising steps of:
• contacting the culture supernatant or the lysate,
• a lectin capable of binding to the sugar chain, wherein the lectin is:
• a protein comprising the amino acid sequence of SEQ ID NO:
• 1 or
• a protein comprising an amino acid sequence in which one amino acid is deleted, substituted, inserted, or added in the amino acid sequence of SEQ ID NO:
• 1 and being capable of binding to a sugar chain represented by the (Formula 1) or (Formula 2), and
• an antibody capable of binding to keratan sulfate, wherein the antibody is an antibody produced by hybridoma R-10G (accession number:
• FERM BP-11301),
• to form a complex comprising the lectin, the sugar chain, and the antibody;
• and
• detecting the complex, wherein the detection of the complex is indicative of the presence of the stem cell.

A method for detecting podocalyxin contained in a culture supernatant or a lysate of cells, the method comprising steps of:

• contacting the culture supernatant or the lysate,
• a lectin capable of binding to a sugar chain represented by (Formula 1) below:
• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule,
• or (Formula 2) below:
• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule, wherein the lectin is:
• a protein comprising the amino acid sequence of SEQ ID NO:
• 1 or
• a protein comprising an amino acid sequence in which one amino acid is deleted, substituted, inserted, or added in the amino acid sequence of SEQ ID NO:
• 1 and being capable of binding to a sugar chain represented by the (Formula 1) or (Formula 2), and
• an antibody capable of binding to keratan sulfate, wherein the antibody is an antibody produced by hybridoma R-10G (accession number:
• FERM BP-11301),
• to form a complex composed of the lectin, podocalyxin, and the antibody;
• and
• detecting the complex, wherein the detection of the complex is indicative of the presence of podocalyxin.

A kit for detecting a stem cell contained among cells by detecting podocalyxin contained in a culture supernatant or a lysate of the cells, the kit comprising:

• a lectin capable of binding to a sugar chain represented by (Formula 1) below:
• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule,
• or (Formula 2) below:
• wherein R1 represents an OH group or any sugar chain and R2 represents an OH group or any sugar chain, protein, lipid, or another molecule, wherein the lectin is:
• a protein comprising the amino acid sequence of SEQ ID NO:
• 1 or
• a protein comprising an amino acid sequence in which one amino acid is deleted, substituted, inserted, or added in the amino acid sequence of SEQ ID NO:
• 1 and being capable of binding to a sugar chain represented by the (Formula 1) or (Formula 2);
• and
• an antibody capable of binding to keratan sulfate, wherein the antibody is an antibody produced by hybridoma R-10G (accession number:
• FERM BP-11301).