Structured illumination microscopy imaging system based on line-scanning spatiotemporal focusing

A structured illumination microscopy imaging system based on line-scanning spatiotemporal focusing, comprising:

• a femtosecond laser;
• an acousto-optic modulator, configured to periodically modulate an intensity of a laser light from the femtosecond laser;
• a line-scanning component, configured to focus the laser light modulated by the acousto-optic modulator to form a line-shaped beam and to scan in a direction perpendicular to the line-shaped beam;
• a chromatic dispersion component, configured to generate spatial chirped laser pulses wherein the chromatic dispersion component comprises a blazed grating, and the blazed grating is configured to disperse the line-shaped beam with broadband to widen a pulse-width in time domain;
• a collimating lens, configured to converge components with different wavelengths dispersed by the chromatic dispersion component to propagate in parallel;
• a microscope component, configured to guide light passing through the collimating lens to illuminate the sample and capture a fluorescence image at a focal plane;
• and
• a synchronous control component, configured to synchronously control the acousto-optic modulator to modulate the intensity of the laser light, the line-scanning component to scan and the microscope component to capture fluorescence images, such that a reconstructed image is obtained according to the images.