Back to results
US 10,539,505 B2Granted
Method for creating a digital fluorescent image
AssigneeCarl Zeiss Microscopy GmbH
InventorsThomas Kalkbrenner, Ralf Wolleschensky
Life of this patent
Estimating the term…
Abstract
In a method for creating a digital fluorescent image, the light emitted per pixel from an object plane is converted into a sequence of amplitudes, each of which is associated with one specific measurement time, the sequence of amplitudes is auto-correlated in a manner that is delayed by at least one time offset, and a specific correlation amplitude, from which a total amplitude is determined, is formed for each of the time offsets.
Claims13
A method for creating a digital fluorescent image, comprising:
- arranging a sample with a fluorescent substance in an object plane of a laser-scanning microscope;
- having excitation light act upon said sample in a time sequence, pixel-by-pixel over a pixel dwell time, so that emission light is generated per pixel which is detected by a detector and converted into a sequence of amplitudes, which are each associated with a respective measuring time and from which a total amplitude is formed;
- combining the total amplitudes of all pixels into exactly one fluorescent image;
- and
- autocorrelating the sequence of amplitudes for each pixel, beginning at a later measuring time is autocorrelated at least once with the same sequence of amplitudes beginning at an earlier measuring time which has a time lag with respect to the later measuring time, for the time lag at least one respective specific correlation amplitude is formed and the total amplitude is determined from the at least one correlation amplitude formed.
2 more elements
Dependencies are inferred from the claim text.