Sample fixation and stabilisation
AssigneeRnassist Ltd.
InventorsAndrew Simon Goldsborough, Malcolm Robert Bates
Life of this patent
Estimating the term…
Abstract
It has surprisingly been discovered that it is possible to stabilize biomolecules such as RNA, DNA and proteins in biological samples such as cells, tissues, biopsies and blood using deep eutectic solvents (DES). It has also been discovered that DES mixtures can be used to fix and preserve cell morphology in biological samples such as tissue blocks, cancer biopsies and whole blood. This invention describes methods to stabilize and preserve biomolecules, whole cells, tissues, blood and biological samples using DES mixtures.
Claims18
A composition comprising a eutectic solvent, wherein said eutectic solvent comprises a first component and a second component;
- wherein said first component is trimethylglycine and said second component is trifluoroacetamide;
- wherein said first component and said second component are in a molar ratio of between about 1:1.5 and about 1:2.5;
- and wherein said first component and said second component together comprise at least about 88% by weight of the composition, wherein said composition further comprises a sample comprising one or more of a biomolecule, RNA, DNA, protein, phosphoprotein, virus, cell, tissue, solid tissue, plasma, serum, brain, cerebrospinal fluid, whole blood, or whole blood comprising a circulating tumor cell.
A composition comprising a eutectic solvent, wherein said eutectic solvent comprises a first component and a second component;
- wherein said first component is trimethylglycine and said second component is trifluoroacetamide;
- wherein said first component and said second component are in a molar ratio of between about 1:1.8 and about 1:2.2;
- and wherein said first component and said second component together comprise at least about 88% by weight of the composition, wherein said composition further comprises a sample comprising one or more of a biomolecule, RNA, DNA, protein, phosphoprotein, virus, cell, tissue, solid tissue, plasma, serum, brain, cerebrospinal fluid, whole blood, or whole blood comprising a circulating tumor cell.
Dependencies are inferred from the claim text.