EGFR assay
AssigneeAbbott Molecular Inc.
InventorsJeffrey D. Wuitschick, Kevin S. Nelson, Shihai Huang, Rupinder Kular, Carolyn Mullen, James Rhoads
Life of this patent
Estimating the term…
Abstract
Provided herein is technology relating to detecting molecular markers relevant to cancer and particularly, but not exclusively, to methods and compositions for quantifying and/or detecting EGFR mRNA and/or EGFRvIII mRNA in biological samples.
Claims6
A composition for detecting EGFRvIII mRNA, the composition comprising a primer comprising a sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 31, a primer comprising a sequence according to SEQ ID NO:
- 2, and a detectably labeled probe comprising a sequence according to SEQ ID NO:
- 3.
A composition for detecting EGFRvIII mRNA and for quantifying total EGFR mRNA, the composition comprising a primer comprising a sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 31, a primer comprising a sequence according to SEQ ID NO:
- 2, a primer comprising a sequence according to SEQ ID NO:
- 7, a primer comprising a sequence according to SEQ ID NO:
- 8, a detectably labeled probe comprising a sequence according to SEQ ID NO:
- 3, and a detectably labeled probe comprising a sequence according to SEQ ID NO:
- 9 or SEQ ID NO:
- 32.
A method for detecting EGFRvIII expression in a sample, the method comprising:
- a) mixing a patient sample with a composition comprising a primer comprising a sequence according to SEQ ID NO:
- 1 or SEQ ID NO:
- 31, a primer comprising a sequence according to SEQ ID NO:
- 2, and a detectably labeled probe comprising a sequence according to SEQ ID NO:
- 3 to provide a RT-PCR reaction mixture;
- b) reverse transcribing EGFRvIII mRNA to provide a EGFRvIII cDNA;
- c) amplifying the EGFRvIII cDNA to provide an EGFRvIII amplicon;
- and
- d) detecting the EGFRvIII amplicon,
- wherein detecting the EGFRvIII amplicon indicates the presence of EGFRvIII expression in the sample.
Dependencies are inferred from the claim text.