Method of amplification of a short tandem repeat locus
AssigneeQiagen GmbH
InventorsDaniel Müller, Sascha Strauss
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Abstract
Short tandem repeat (STR) markers are genetic elements that are frequently used in the fields of forensic analysis, paternity determination and detection of genetic diseases and cancers. Such analysis involves the amplification of STR loci. Technically, this can be challenging due to sequence variations in the flanking regions of the locus. In the case of SE33, previous amplification efforts have failed. The present invention describes a set of primers for the amplification of SE33 and a method for the analysis of the presence and/or level of SE33, also in combination with other STRs.
Claims10
A method for the detection of the presence and/or level of the SE33 marker comprising the following steps:
- i. mixing a sample with two nucleic acid oligonucleotides, wherein one nucleic acid is a forward primer consisting of a sequence selected from the group of,
- a) SEQ ID NO:
- 1 and,
- b) a sequence that is at least 75% identical to SEQ ID NO:
- 1, wherein said nucleic acid that is at least 75% identical to SEQ ID NO:
- 1 has at least 2 terminal 3′-prime nucleotides that are identical to the 2 terminal 3′-prime nucleotides of SEQ ID NO:
- 1 and, wherein, said nucleic acid may also optionally be 1 to 5 nucleotides shorter or longer at its 5′ end and the other is a reverse primer suitable to form an amplification product when used with said forward primer in an amplification reaction,
- ii. performing an amplification reaction and
- iii. detection of the presence and/or level of SE33.
Dependencies are inferred from the claim text.