US 10,538,801 B2 · Method for determining whether or not test sample contains exserohilum phytopathogenic fungus

Abstract

The present invention provides a method for determining whether or not a test sample contains an Exserohilum phytopathogenic fungus. The method comprises: (a) putting the test sample on a front surface of a cellulose film having no through hole; (b) leaving the test sample at rest for a predetermined time; (c) observing a back surface of the cellulose film; and (d) determining that the test sample contains the Exserohilum phytopathogenic fungus, if a fungus which has penetrated the cellulose film is found on the back surface of the cellulose film in the step (c). The method further comprises a step of supplying a culture medium to the test sample before the step (b). The culture medium is a lactose casein hydrolysate agar medium containing carbendazim, captan, streptomycin sulfate, and neomycin sulfate. Alternatively, in the step (b), the test sample is left at rest while the back surface of the cellulose film is in contact with the culture medium.

Claims22

Dependent claims

A method for determining whether or not a test sample contains an Exserohilum phytopathogenic fungus, the method comprising:

(a) putting the test sample on a front surface of a cellulose film having no through hole and which forms a portion of a bottom of a container;
(b) incubating the test sample at room temperature for a predetermined time after step (a);
(c) inspecting and quantifying a back surface of the cellulose film outside of the container after step (b) for fungi hyphae;
and
(d) identifying the Exserohilum phytopathogenic fungus from the test sample if a fungus which has penetrated the cellulose film is found on the back surface of the cellulose film in step (c);
wherein the method further comprises a step of supplying a culture medium to the test sample before step (b);
and the culture medium is a lactose casein hydrolysate agar medium containing:
carbendazim;
captan;
streptomycin sulfate;
and neomycin sulfate,
wherein the predetermined time is greater than or equal to 24 hours, and wherein the back surface of the cellulose film is an external surface of the container.

9 more elements

Dependencies are inferred from the claim text.

Method for determining whether or not test sample contains exserohilum phytopathogenic fungus

Life of this patent

Method for determining whether or not test sample contains exserohilum phytopathogenic fungus

Life of this patent

Abstract

Claims22