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US 10,538,793 B2Granted
ω3 fatty-acid desaturase and method for producing eicosapentaenoic acid
AssigneesKyoto University, Nisshin Pharma Inc.
InventorsJun Ogawa, Akinori Ando, Eiji Sakuradani, Sakayu Shimizu, Shigeru Hiramoto, Masataka Harata, Yuki Takemoto
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Abstract
Provided is ω3 desaturase having high enzymatic activity even at normal temperature. A polypeptide which consists of an amino acid sequence having an identity of 80% or more with the amino acid sequence represented by SEQ ID NO: 2 and has ω3 desaturation activity on C20 fatty acid, and a gene thereof.
Claims11
A polypeptide, consisting of an amino acid sequence of SEQ ID NO:
- 2, except that at least 5 and no more than 20 amino acids of the polypeptide are modified relative to the amino acid sequence of SEQ ID NO:
- 2 by mutations selected from the group consisting of a deletion, a substitution, an insertion and an addition,
- wherein said polypeptide has ω3 desaturation activity on a C20 fatty acid.
A polypeptide, consisting of an amino acid sequence of SEQ ID NO:
- 4, except that at least 5 and no more than 20 amino acids of the polypeptide are modified relative to the amino acid sequence of SEQ ID NO:
- 4 by mutations selected from the group consisting of a deletion, a substitution, an insertion and an addition,
- wherein said polypeptide has ω3 desaturation activity on a C20 fatty acid.
A cDNA polynucleotide, consisting of the following nucleotide sequence:
- (a) the nucleotide sequence of SEQ ID NO:
- 1;
- (b) a nucleotide sequence having an identity of 95% or more with the nucleotide sequence of SEQ ID NO:
- 1;
- (c) a nucleotide sequence consisting of SEQ ID NO:
- 1, except that at least 1 and no more than 20 nucleotides of the nucleotide sequence are modified relative to SEQ ID NO:
- 1 by mutation selected from the group consisting of a deletion, a substitution, an insertion and an addition;
- (d) the nucleotide sequence of SEQ ID NO:
- 3;
- (e) a nucleotide sequence having an identity of 95% or more with the nucleotide sequence of SEQ ID NO:
- 3;
- (f) a nucleotide sequence consisting of SEQ ID NO:
- 3, except that at least 1 and no more than 20 nucleotides of the nucleotide sequence are modified relative to SEQ ID NO:
- 3 by mutation selected from the group consisting of a deletion, a substitution, an insertion and an addition;
- or
- (g) a nucleotide sequence obtained by optimizing a codon of any one of the nucleotide sequences of (a) to (f);
- wherein the cDNA polynucleotide encodes a polypeptide having ω3 desaturation activity on a C20 fatty acid.
14 more elements
Dependencies are inferred from the claim text.