Lyophilized lentiviral vector particles, compositions and methods

A method of administering a lentiviral particle to a human subject, comprising:

• a) making lyophilized lentiviral vector particles by a method comprising:
• i) providing a cell supernatant comprising lentiviral vector particles,
• ii) purifying the lentiviral vector particles in the supernatant,
• iii) concentrating the purified lentiviral vector particles,
• iv) freezing the concentrated lentiviral vector particles to provide frozen lentiviral vector particles,
• v) lyophilizing the frozen lentiviral vector particles in the presence of one or more sugars to provide lyophilized lentiviral vector particles, and
• vi) storing the lyophilized lentiviral vector particles for at least three days, wherein the lyophilized lentiviral vector particles retain at least 80% of their titer after freezing viral titer when stored for 45 days at -20° C.;
• b) reconstituting the lyophilized lentiviral vector particles in an aqueous medium for administration;
• and
• c) administering the reconstituted lentiviral vector particles to the human subject.