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US 10,538,784 B2Granted
Plant virus movement proteins and methods of using the same
AssigneeMolecular Transfer, Inc.
InventorsGulilat Gebeyehu, Joel Jessee
Life of this patent
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Abstract
Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.
Claims5
A transfection complex comprising a combination of a cationic lipid, a neutral lipid, and a peptide, wherein:
- the cationic lipid is a compound of Formula 1,
- wherein:
- Z═(CH2)q, (CH2)q—N(R3)—(CH2)q, (CH2)q—S—S—(CH2)q, or (CH2)q—O—(CH2)q, wherein q=1-3 and R3 ═CH3;
- and
- i) R1═R2═C16H31, X═O, W1═H;
- W2═CH2—O—P(═O)(OMe)-O—CH2CH2—NH—C(═O)-spermine, y=0 (DPePE-Sp-OMe);
- or
- ii) W1═W2═CH2—N(R3R4), R1═R2═C16H31, X═O;
- R3═CH3, R4═—C(═O)—C(NH2)—CH2—(CH2)n—C(═NH)—NH2, n=0-6, y=0 (DPB-Arg);
- and
- the neutral lipid is selected from dioleoylphosphatidylethanolamine (DOPE), diphytanoylphosphatidylethanolamine (DPhPE), and 3-oleyloxy-2-hydroxy-1-acetamidopropane (HOAP);
- and
- the peptide is selected from:
- a) SEQ ID NO:507 covalently linked to SEQ ID NO:109 (Peptide A);
- b) SEQ ID NO:540 (Peptide B);
- and
- c) SEQ ID NO:350 covalently linked to SEQ ID NO:109 (Peptide C).
14 more elements
Dependencies are inferred from the claim text.