Methods for genetic control of insect infestations in plants and compositions thereof

A double stranded ribonucleotide sequence produced from expression of a recombinant polynucleotide molecule, the recombinant polynucleotide sequence comprising a first, a second and a third polynucleotide sequence, wherein the third polynucleotide sequence is linked to the first polynucleotide sequence by the second polynucleotide sequence, and wherein the third polynucleotide sequence is substantially the reverse complement of the first polynucleotide sequence such that the first and the third polynucleotide sequences hybridize when transcribed into a ribonucleic acid to form the double stranded ribonucleotide molecule stabilized by the linked second ribonucleotide sequence, and wherein the first polynucleotide sequence comprises a recombinant polynucleotide selected from the group consisting of:

• (a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO:759, 819, 825, 826, or 827;
• (b) a polynucleotide comprising at least 95% sequence identity to the nucleic acid sequence of SEQ ID NO:759, 819, 825, 826, or 827;
• (c) a polynucleotide comprising a fragment of at least 21 contiguous nucleotides of the nucleic acid sequence of SEQ ID NO:759, 819, 825, 826, or 827;
• and
• (d) a polynucleotide comprising a complement of the sequence of (a), (b), or (c);
• wherein ingestion of the double stranded ribonucleotide sequence by a coleopteran plant pest inhibits growth of said coleopteran plant pest.