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US 10,538,774 B2Granted
Methods and means to modify a plant genome
AssigneeBASF Agricultural Solutions Seed US LLC
InventorKathleen D'Halluin
Life of this patent
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Abstract
Methods and means are provided to modify in a targeted manner the genome of a plant in close proximity to an existing elite event using a double stranded DNA break inducing enzyme. Also provided are plants, in particular cotton plants showing tolerance to a field dose of at least 1X of at least one HPPD inhibitor, and methods for making such plants.
Claims19
A method for modifying the genome of a plant cell at a predefined site comprising:
- a. inducing a double stranded DNA break within 1 kb of or at said predefined site, said double stranded break being induced by the introduction into said cell of a double stranded DNA break inducing (DSBI) enzyme which recognizes a recognition sequence within 1 kb of or at said predefined site;
- and
- b. selecting a plant cell wherein said double stranded DNA break has been repaired resulting in a modification in the genome at said predefined site, wherein said modification is
- i. a replacement of at least one nucleotide;
- ii. a deletion of at least one nucleotide;
- iii. an insertion of at least one nucleotide;
- or
- iv. any combination of i.-iii.;
- wherein said recognition sequence comprises the nucleotide sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
8 more elements
Dependencies are inferred from the claim text.