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US 10,538,760 B2Granted
Methods for multiplexing recombinase polymerase amplification
AssigneeAlere San Diego, Inc.
InventorsOlaf Piepenburg, Colin H. Williams, Niall A. Armes
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Abstract
This disclosure provides for methods and reagents for rapid multiplex RPA reactions and improved methods for detection of multiplex RPA reaction products. In addition, the disclosure provides new methods for eliminating carryover contamination between RPA processes.
Claims18
A device comprising:
- (a) a reaction chamber containing a dried reagent composition for performing a recombinase polymerase amplification (RPA), said dried reagent
- composition comprising:
- (i) at least one recombinase;
- (ii) at least one polymerase;
- (iii) a first nucleic acid primer and a second nucleic acid primer, wherein said first nucleic acid primer comprises a first detectable label;
- (iv) a third extension blocked primer that comprises an elongation blocking group at the 3′ end and an internal tetrahydrofuran (THF) residue, and wherein said third extension blocked primer comprises a second detectable label that is different than said first detectable label;
- and
- (v) at least one nuclease;
- and
- (b) a lateral flow strip configured to detect the amplified product.
7 more elements
Dependencies are inferred from the claim text.