Alpha-amylases and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsGuillermo Coward-Kelly, Shiro Fukuyama, Noriko Tsutsumi, Keiichi Ayabe
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Abstract
The present disclosure relates to isolated polypeptides having alpha-amylase activity, polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing the polypeptides, and method of using polypeptides, including in ethanol production processes.
Claims20
A recombinant host cell comprising a polynucleotide encoding a polypeptide having alpha-amylase activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide, and wherein the polypeptide is selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of the mature polypeptide of the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:4, or SEQ ID NO:6;
- (b) a polypeptide comprising the amino acid sequence having at least 90% sequence identity to the mature polypeptide of the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:4, or SEQ ID NO:6;
- and
- (c) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:3, or SEQ ID NO:5.
A method of producing a polypeptide having alpha-amylase activity, comprising:
- (a) cultivating a host cell under conditions conducive for production of the polypeptide, wherein the host cell comprises a polynucleotide encoding a polypeptide having alpha-amylase activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide, and wherein the polypeptide is selected from the group consisting of:
- (i) a polypeptide comprising the amino acid sequence of the mature polypeptide of the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:4, or SEQ ID NO:6;
- (ii) a polypeptide comprising the amino acid sequence having at least 90% sequence identity to the mature polypeptide of the amino acid sequence of SEQ ID NO:
- 2, SEQ ID NO:4, or SEQ ID NO:6;
- and
- (iii) a polypeptide encoded by a polynucleotide having at least 90% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:3, or SEQ ID NO:5;
- and
- (b) recovering the polypeptide.
Dependencies are inferred from the claim text.