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AssigneeInstitute of Microbiology, Chinese Academy of Sciences
InventorsWenjun Liu, Weinan Zheng, Jing Li
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A recombinant virus is obtained by mutating a codon that encodes a tyrosine residue at position 385 of NP protein in the genome of influenza A virus to a codon of phenylalanine residue. The virus WSN-Y385F is a temperature-sensitive virus that can normally replicate and survive at 37° C., and cannot normally replicate and cannot survive at 33° C. Phosphorylation of a NP protein of influenza A virus can be inhibited by mutating an amino acid residue at position 385 from N terminal of the NP protein of influenza A virus, from a tyrosine to a phenylalanine. The recombinant virus can be used in analyzing mechanisms of infection by influenza virus, and in connection with methods of prevention and treatment of infection by influenza virus.
A recombinant influenza A virus, comprising a genome with a nucleoprotein (NP) in which a codon that encodes a tyrosine residue at a position corresponding to position 385 in SEQ ID NO:
An influenza A nucleoprotein protein having a phenylalanine residue at a position corresponding to position 385 in SEQ ID NO:
A method for preparing a recombinant influenza A virus comprising:
A method of removing a phosphorylation site of a NP protein of influenza A virus comprising mutating an amino acid residue at a position corresponding to position 385 of SEQ ID NO:
A plasmid combination consisting of the plasmid pHH21-PA, plasmid pHH21-PB1, plasmid pHH21-PB2, plasmid pHH21-HA, plasmid pHH21-NA, plasmid pHH21-M, plasmid pHH21-NS, plasmid pcDNA3.0-PA, plasmid pcDNA3.0-PB1, plasmid pcDNA3.0-PB2, recombinant plasmid pHH21-NP-Y385F and recombinant plasmid pcDNA3.0-NP-Y385F.
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