Megakaryocytic particles and microparticles for cell therapy and fate modification of stem and progenitor cells

An in vitro method to generate particles for platelet function comprising the steps of:

• a. generating cultured cells selected from the group consisting of megakaryocytes and immature megakaryocyte cells;
• b. exposing said cultured cells to a biomechanical stress that generates at least one particle for platelet function;
• c. collecting said particles from a supernatant of the cultured cells in step b;
• d. loading said particles obtained from step c with native cellular content(s) of said cultured cells;
• e. providing said loaded particles to a co-culture with at least one target cell to provide a transfer of said cellular content(s) to said target cells, wherein said target cell is selected from the group consisting of hematopoietic stem cells, and progenitor cells;
• wherein said co-culture with said target cells is performed in the presence of a culture medium lacking thrombopoietin;
• and
• wherein said transfer enables effective differentiation of said target cells and production of additional particles for platelet function selected from the group consisting of megakaryocyte microparticles, proplatelets, preplatelets, platelet-like particles and megakaryocyte extracellular vesicles.