Pomegranate derived cell culture and methods for preparing and using the same

A scale-up process for the in vitro production of a pomegranate fruit cell culture of pomegranate aril cells grown comprising:

• growing pomegranate aril cells in a flask;
• inoculating the pomegranate aril cells from the flask into a first bioreactor;
• inoculating the pomegranate aril cells from the first bioreactor into a second bioreactor;
• and
• harvesting the pomegranate aril cells from the last bioreactor;
• wherein the second bioreactor is a last bioreactor or an intermediate bioreactor;
• wherein at least one of the first and the second bioreactor is disposable;
• wherein the pomegranate aril cells harvested from the last bioreactor are dried;
• wherein the size of each bioreactor used in the process is larger than the one in which the pomegranate aril cells were previously grown;
• wherein the pomegranate aril cells harvested from the last bioreactor comprise a total polyphenol concentration of between 100,000-700,000 mg/kg dry weight powder;
• wherein the pomegranate aril cells are grown in a growth medium comprising Gamborg B5 medium at pH of 5.4-5.8 comprising 2-4% sucrose, 0.2-0.3 g/L casein hydrolysate, 0.05-0.15 g/L myo inositol, 0.05-0.15 mg/L 1-naphthaleneacetic acid (NAA) and, 0.1-0.3 mg/L kinetin, 25-45 mM KNOB, 1-15 mM MgSO4 or 5-35 mM MgNO3 and 1 mM NaH2PO4.