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US 10,538,736 B2Granted
Conditioned medium and extracellular matrix compositions from cells cultured under hypoxic conditions
AssigneeHistogen, Inc.
InventorsGail K. Naughton, Francis C. Zeigler, Mark Baumgartner, Kyle Nickey
Life of this patent
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Abstract
The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
Claims7
A method of producing a composition for improvement of a tissue in a subject comprising:
- culturing fibroblast cells under hypoxic conditions on microcarrier beads or a three dimensional surface in a suitable growth medium, under 1-5% oxygen for at least 2 weeks, thereby producing multipotent stem cells, wherein the multipotent stem cells produce and secrete into the growth medium a composition that promotes repair and regeneration of damaged tissue when administered to the region of tissue in need of repair in the subject, and
- collecting the growth medium after the 2 weeks,
- thereby producing the composition.
Dependencies are inferred from the claim text.