Multispecific antibody platform and related methods
AssigneeBison Therapeutics Inc.
InventorWenjun Zhang
Life of this patent
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Abstract
Novel multispecific antibody platform, multispecific antibodies and a method to make the antibodies is disclosed here. The CH3 domain harboring polypeptide chains of the Fc-regions are modified by increasing the positive charge of one chain and negative charge of another chain. Heterodimer formation in cotransfection with the modified chains is comparable to formation of wild type antibodies when same cell line is transfected with wild type polypeptides. Moreover, the modification provides a simple one step purification of the heterodimers.
Claims12
An isolated polypeptide comprising:
- a CH3 domain, said CH3 domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:1, 3, 3, 5, 7, 9, 11, 13, 15, 17, 21, 23, 27, 27, 29, 31, 33 and 35.
A multispecific heterodimeric antibody comprising:
- a first immunoglobulin heavy chain comprising a first CH3 domain and a second immunoglobulin heavy chain comprising a second CH3 domain, wherein the first immunoglobulin heavy chain comprising the first CH3 domain has an amino acid sequence selected from the group consisting of SEQ ID No 3, 5, 9, 13, 17, 21, 25, 31, 35, and wherein the second immunoglobulin heavy chain comprising the second CH3 domain has an amino acid sequence selected from the group consisting of SEQ ID No 1, 7, 11, 15, 19, 23, 27, 29, 33.
An antibody comprising:
- a first immunoglobulin heavy chain and a second immunoglobulin heavy chain, wherein the first immunoglobulin heavy chain comprises one of the following triple amino acid substitutions (P395K, P396K, V397K), (P395K, P396K, V397C) or (P395R, P396R, V397R), wherein the numbering is according to the EU numbering scheme;
- and the second immunoglobulin heavy chain comprises one of the following triple amino acid substitutions (T394D, P395D, P396D), (T394C, P395D, P396D) or (T394E, P395E, P396E), wherein the numbering is according to the EU numbering scheme, and vice versa.
Dependencies are inferred from the claim text.