US 10,538,564 B2Granted

Peptides having osteoblast growth-promoting activity and use thereof

AssigneePharma Foods International Co., Ltd.

InventorsChihiro Yamazaki, Ji-Yeong An, Seiyu Harada, Kazuya Watabe, Mujo Kim

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention provides a novel peptide having bone formation-promoting effect and chondrocyte growth-promoting effect, in particular, a peptide having osteoblast growth-promoting activity, having 100 amino acid residues or less comprising an amino acid sequence selected from (a) Val-Asn-Pro-Glu-Ser-Glu-Glu-Glu-Asp-Glu-Ser-Ser-Pro-Tyr-Glu (SEQ ID NO: 1), (b) an amino acid sequence derived from the amino acid sequence (a) by conservative substitution or deletion of 1 to 3 amino acids, and (c) an amino acid sequence consisting of at least four contiguous amino acids of the amino acid sequence (a) or (b), or a derivative thereof or a salt thereof.

Claims5

Dependent claims

A method for promoting bone formation, the method comprising administering, to a mammal in need thereof, an effective amount of a peptide or a salt thereof having osteoblast growth-promoting activity, wherein said peptide consists of an amino acid sequence selected from the group consisting of:

(SEQ ID NO:
1)
(i)
Val-Asn-Pro-Glu-Ser-Glu-Glu-Glu-Asp-Glu-Ser-
Ser-Pro-Tyr-Glu,
(SEQ ID NO:
2)
(ii)
Val-Asn-Pro-Glu-Ser-Glu-Glu-Glu,
(SEQ ID NO:
3)
(iii)
Val-Asn-Pro-Glu-Ser-Glu-Glu,
(SEQ ID NO:
4)
(iv)
Pro-Glu-Ser-Glu-Glu,
(SEQ ID NO:
5)
(v)
Asp-Glu-Ser-Ser-Pro-Tyr-Glu,
and
(SEQ ID NO:
6)
(vi)
Glu-Asp-Glu-Ser-Ser-Pro-Tyr-Glu,
wherein the peptide has at least one phosphorylated serine, and
wherein the peptide promotes chondrocyte growth, induces chondrogenic cell differentiation, promotes hyaluronic acid production, promotes mesenchymal stem cell growth, induces mesenchymal stem cell differentiation, or increases the serum level of insulin-like growth factor (IGF-1) in the mammal.

25 more elements

Dependencies are inferred from the claim text.

Facts

Application: 15/121,471Filed 25 Feb 2015
Priority: JP 2014-03424025 Feb 2014
Published: US 2016/0362466 A115 Dec 2016
Granted: 21 Jan 2020
Term ends: Not estimatedThe source records don’t support an estimate.

Classification24 codes

C07K 14/51
Growth factors; Growth regulators: bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
A23L 33/18
Amino acids, peptides or proteins: peptides; Protein hydrolysates
A61K 8/64
Containing organic compounds: proteins; Peptides; Derivatives or degradation products thereof
A61K 38/04
Medicinal preparations containing peptides: peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof