Centrifuge tube assembly for separating, concentrating and aspirating constituents of a fluid product
OwnerPatrick Pennieinventor
Life of this patent
Estimating the term…
Abstract
A centrifuge tube assembly includes an elongate tubular receptacle having upper and lower ends and a side wall that extends between the ends. A cap attached to the receptacle carries a common inlet/outlet port formed therethrough for communication with an interior chamber of the tubular receptacle. A liquid impermeable piston is longitudinally movable through the receptacle chamber and sealably interengages an interior wall of the receptacle. The piston separates upper and lower regions of the chamber, which respectively communicate with the inlet/outlet port and a pressure neutralizing vent. Biological products are introduced into and aspirated from the receptacle through the inlet/outlet port.
Claims19
A centrifuge tube assembly for separating and aspirating constituent components of a fluid biological product, said assembly comprising:
- a receptacle having an interior chamber for receiving the fluid biological product therein;
- a piston mounted within said chamber and being slidable through said chamber while maintaining sealing engagement with an interior surface of a side wall of said receptacle;
- a common inlet and outlet port supported by said receptacle and communicating with a first region of said chamber on one side of said piston;
- a vent supported by said receptacle and communicating with a second region of said chamber on an opposite side of said piston;
- and
- hypodermic injecting and aspirating syringes, which are selectively interengaged with said common inlet and outlet port outside of said receptacle;
- the fluid biological product being injected by said injecting syringe through said common port into said first region of said chamber and said receptacle being centrifuged to separate the fluid product into the constituent components thereof, which components are disposed in respective fluid layers in said chamber, whereby one or more of the fluid layers may be aspirated through said common inlet and outlet port by said aspirating syringe, said vent neutralizing pressure within said second region of said receptacle chamber as fluid is injected into said chamber and as constituent components are aspirated from said chamber through said common inlet and outlet port.
A centrifuge tube assembly for use selectively and operatively with a hypodermic injecting syringe and a hypodermic aspirating syringe to separate and aspirate constituent components of a fluid biological product, said centrifuge tube assembly comprising:
- a receptacle having an interior chamber for receiving a fluid biological product therein;
- a piston mounted within said chamber and being slidable through said chamber while maintaining sealing engagement with an interior surface of a side wall of said receptacle;
- a female common inlet and outlet port supported by said receptacle in communication with a first region of said chamber located on one side of said piston, said female common inlet and outlet port configured for selectively receiving the injecting syringe and the aspirating syringe;
- and
- a vent supported by said receptacle in communication with a second region of said chamber located on an opposite, second side of said piston;
- fluid biological product being injected through said female common inlet and outlet port into said first chamber region and said receptacle being centrifuged to separate the fluid product into constituent components thereof, which components are disposed in respective layers in said first chamber region;
- whereby one or more of the fluid layers may be aspirated through said female common inlet and outlet port, said vent neutralizing air pressure within said second chamber region as fluid is injected into and as constituent components are aspirated from said first chamber region.
A method of separating fluid biological product into constituent components using a centrifuge tube assembly, which assembly includes an elongate tubular receptacle having an interior chamber, a piston mounted for sliding within the receptacle chamber while maintaining sealing engagement with an interior surface of a side wall thereof, a common inlet and outlet port supported by the receptacle and configured for injecting fluid biological product into and aspirating constituent components from a first region of the receptacle on one side of the piston, and a vent supported by the receptacle and communicating with a second region of the chamber on an opposite side of the piston, said method comprising:
- injecting the fluid biological product into the receptacle through the common inlet and outlet port into the first region of the chamber such that the hydraulic pressure of the injected fluid drives said piston through the receptacle and until said receptacle is filled to a selected level by the biological product;
- closing the common inlet and outlet port;
- centrifuging the receptacle to separate the fluid biological product into constituent components;
- opening the common inlet and outlet port;
- and
- aspirating one or more of the constituent components from the first chamber through the common inlet and outlet port such that a suction is generated to draw said sealing piston through the receptacle in the direction of the common inlet and outlet port, wherein the vent neutralizes pressure within the second chamber region as fluid is introduced into and constituent components are aspirated from said first chamber region.
Dependencies are inferred from the claim text.