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US 10,537,887 B2Granted
Adsorbent composition and use thereof
AssigneesBoehringer Ingelheim Rcv GmbH & Co KG, Sandoz Ltd.
InventorsRainer Hahn, Alois Jungbauer, Alexandru Trefilov, Moritz Imendoerffer
Life of this patent
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Abstract
The present invention provides a novel adsorbent composition for recovering biomolecules from a fluid. The composition comprises positively and negatively charged microparticles in the form of ground particles. The adsorbent is particularly useful for purification of biomolecules from the cell culture.
Claims18
A composition consisting essentially of positively charged microparticles and negatively charged microparticles,
- wherein the positively charged microparticles comprise a ground polymeric anion-exchange resin, and wherein the negatively charged microparticles comprise a ground polymeric cation exchange resin,
- wherein the anion-exchange resin and the cation exchange resin are polystyrene-based, Hydroxyethyl methacrylate (HEMA)-based, dimethylamino ethylmethacrylate (DMAEMA)-based, dimethylamino ethylmethacrylate (pDMAEMA), or methacrylic acid (MAA)-based, and
- wherein the microparticles have an average particle size of less than about 5 μm.
A method of obtaining a biomolecule from a biological fluid comprising said biomolecule comprising:
- a) adding positively charged microparticles comprising a ground polymeric anion-exchange resin and adding negatively charged microparticles comprising a ground polymeric cation-exchange resin to the biological fluid,
- b) allowing the microparticles to form flocs,
- c) removing the flocs from the biological fluid, and
- d) recovering the biomolecule;
- wherein the anion-exchange resin and the cation exchange resin are polystyrene-based, Hydroxyethyl methacrylate (HEMA)-based, dimethylamino ethylmethacrylate (DMAEMA)-based, dimethylamino ethylmethacrylate (pDMAEMA), or methacrylic acid (MAA)-based.
2 more elements
A composition comprising
- (a) a biological fluid comprising biomolecules,
- (b) positively charged microparticles comprising a ground polymeric anion-exchange resin, and
- c) negatively charged microparticles comprising a ground polymeric cation-exchange resin;
- and
- wherein the microparticles have an average particle size of less than about 5 μm.
2 more elements
Dependencies are inferred from the claim text.