Preparation method of hydrogel based on decellularized tissue using supercritical fluid-organic solvent system

A method for preparing a decellularized tissue-based hydrogel, comprising:

• (a-1) a step of freeze-drying a biological tissue and immersing the same in an organic solvent for 5-20 hours;
• (a) a step of decellularizing a biological tissue by bringing the same to contact with a supercritical fluid and an organic solvent at the same time;
• (b) a step of washing the decellularized tissue;
• (c) a step of preparing a decellularized tissue solution by mixing the washed decellularized tissue with one selected from an enzyme solution, an acidic solution and a mixture thereof, wherein,
• the decellularized tissue solution is prepared by mixing the washed decellularized tissue and the acidic solution at a weight ratio of 10-50:1, then, mixing with a collagen solution,
• the collagen solution comprises collagen and deionized water at a weight ratio of 1-5:1, and
• a volume ratio of the collagen solution and the decellularized tissue solution is 5:5;
• (d) a step of titrating the decellularized tissue solution to pH 5.5-7.8 by treating with a basic solution;
• and
• (e) a step of allowing the titrated decellularized tissue solution to stand at 30-40° C.