Subunit vaccine delivery platform for robust humoral and cellular immune responses
AssigneeCornell University
InventorsDavid A. Putnam, Joseph Rosenthal, Chung-Jr Huang, Matthew DeLisa, Susana Mendez
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Abstract
The present invention relates to a probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides. Probiotic-derived vesicles displaying this fusion protein as well as methods of inducing an immune response using the probiotic cells or vesicles are also disclosed.
Claims53
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic food allergy proteins or peptides.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic viral proteins or peptides.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the probiotic cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic intracellular bacterial proteins or peptides.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic proteins or peptides wherein the cell is Escherichia coli Nissle, and wherein the transport protein is ClyA.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic viral proteins or peptides, wherein the cell is Escherichia coli Nissle, and wherein the transport protein is ClyA.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic food allergy proteins or peptides, wherein the cell is Escherichia coli Nissle, and wherein the transport protein is ClyA.
A probiotic cell transformed with a construct suitable to overexpress and display on the surface of the cell a fusion protein comprising at least a portion of a transport protein coupled to at least a portion of one or more antigenic intracellular bacterial proteins or peptides, wherein the cell is Escherichia coli Nissle, and wherein the transport protein is ClyA.
Dependencies are inferred from the claim text.