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US 10,537,625 B2Granted
Micro-RNA-155 enhances the efficacy of dendritic cell vaccine for cancer
AssigneeUniversity of South Carolina
InventorDaping Fan
Life of this patent
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Abstract
Engineered dendritic cell vaccines, and methods of forming and applying same, that may be used as effective immunotherapies for cancers.
Claims9
A method for suppressing tumor growth:
- amplifying a DNA fragment containing a microRNA;
- sub-cloning the microRNA segment into a delivery vehicle;
- delivering the micro-RNA segment to a cell via the delivery vehicle;
- whereby delivery of the micro-RNA segment causes the cell to overexpress the micro-RNA;
- injecting the cell into a tumor microenvironment;
- wherein overexpression of the micro-RNA segment by the cell suppresses tumor growth;
- and
- wherein the tumor being suppressed is a breast cancer tumor wherein the microRNA segment comprises micro-RNA 155, and wherein the cell receiving the micro-RNA segment is a dendritic cell.
5 more elements
A method for using a lentiviral vector to suppress tumor growth:
- amplifying a DNA fragment containing a microRNA stem loop;
- sub-cloning the DNA fragment containing the microRNA stem loop into a lentiviral vector;
- delivering the lentiviral vector to a cell;
- introducing the cell into a tumor microenvironment;
- whereby delivery of the lentiviral vector containing the micro-RNA causes dendritic cell maturation followed by T-cell activation to suppress tumor growth;
- and
- wherein the tumor being suppressed is a breast cancer tumor wherein the microRNA stem loop comprises micro-RNA 155, and wherein the cell is a dendritic cell.
4 more elements
Dependencies are inferred from the claim text.