Gas-filled microvesicles for use as vaccine
AssigneeBracco Suisse SA
InventorsGilles Bioley, Philippe Bussat, Blaise Corthesy, Anne Lassus
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Abstract
Gas-filled microvesicles comprising an antigen bound thereto and to aqueous suspensions containing said microvesicles, for use in immunomodulating formulations, in particular as a vaccine. The antigen is covalently bound to a component of the microvesicles envelope. The microvesicles of the invention, comprising a molar excess of fatty acids in the stabilizing envelope, are particularly effective in the uptake by antigen-presenting cells, in particular dendritic cells.
Claims16
A pharmaceutical formulation comprising an aqueous suspension of gas-filled microvesicles with a stabilizing envelope, said stabilizing envelope comprising (a) an antigen covalently bound to an amphiphilic component forming said stabilizing envelope, (b) a phospholipid, and (c) a fatty acid, with a molar ratio between the phospholipid and the fatty acid lower than 25/75.
A method of using a pharmaceutical formulation in an immunomodulating treatment, wherein the pharmaceutical formulation comprises an aqueous suspension of gas-filled microvesicles with a stabilizing envelope, wherein said stabilizing envelope comprises (a) an antigen covalently bound to an amphiphilic component forming said stabilizing envelope, (b) a phospholipid, and (c) a fatty acid, and wherein the phospholipid and the fatty acid has a molar ratio of lower than 25/75;
- the method comprising administering the pharmaceutical formulation.
A precursor of a pharmaceutical formulation comprising an aqueous suspension of gas-filled microvesicles with a stabilizing envelope that comprises (a) an antigen covalently bound to an amphiphilic component forming said stabilizing envelope, (b) a phospholipid, and (c) a fatty acid, wherein the phospholipid and the fatty acid has a molar ratio of lower than 25/75;
- wherein the precursor is in a form of a freeze-dried preparation reconstitutable in a physiologically acceptable aqueous carrier in a presence of a physiologically acceptable gas.
Dependencies are inferred from the claim text.