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US 10,537,596 B2Granted
Bone marrow adipose portion isolation device and methods
AssigneeRegenexx, LLC
InventorsChristopher Centeno, Ryan Dregalla, Nicolette Lyons, Patrick Reischling
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Abstract
The embodiments disclosed herein generally relate to systems, devices and methods for the fractionation, isolation, extraction and processing of the adipose supernatant layer of a bone marrow aspirate. In particular, the various embodiments relate to systems devices and methods of obtaining, utilizing and processing the adipose supernatant layer of a bone marrow aspirate as a source of mesenchymal stem cells.
Claims12
A method of processing bone marrow aspirate comprising:
- providing a device for processing bone marrow aspirate comprising:
- a first chamber;
- a second chamber in fluid communication with the first chamber;
- and
- a mechanical emulsification system in fluid communication with the second chamber;
- fractionating bone marrow aspirate within the first chamber of the device into layers including an adipose layer supernatant;
- collecting the adipose layer supernatant from the processed bone marrow aspirate in the second chamber;
- and
- emulsifying the adipose layer supernatant in the mechanical emulsification system.
6 more elements
Dependencies are inferred from the claim text.