Targeted transplantation of mitochondria to hepatocytes
AssigneeUniversity of Connecticut
InventorsGeorge Y Wu, Catherine H Wu, Nidhi Gupta
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Abstract
The present disclosure relates to, inter alia, compositions and kits comprising an asialoglycoprotein covalently attached to a polycation, and functional mammalian mitochondria that are at least partially purified and are electrostatically bound to the AsG-polycation molecule; as well as methods of their preparation and use.
Claims22
A pharmaceutical composition comprising:
- an asialoglycoprotein (AsG) covalently attached to a polycation;
- and
- functional mammalian mitochondria that are at least partially purified;
- wherein the AsG, covalently attached to the polycation, is complexed electrostatically to the mitochondria.
A kit comprising a pharmaceutical composition that comprises an asialoglycoprotein (AsG) covalently attached to a polycation;
- functional mammalian mitochondria, at least partially purified;
- and instruction for using said composition to treat a liver disease.
A method of making a composition comprising:
- at least partially purifying functional mitochondria from a cell;
- allowing an AsG to be covalently attached to a polycation, wherein the polycation is highly positively charged and is selected from the group of polycations consisting of polylysine (PL), polyarginine, and polyornithine;
- and
- allowing the AsG, covalently attached to the polycation, to complex electrostatically to the mitochondria.
A method of transplanting mitochondria into a hepatocyte, comprising providing a composition comprising functional mammalian mitochondria complexed with AsG-PL electrostatically, and an endosomolytic agent that is covalently attached to a separate AsG by a cleavable bond;
- and delivering said composition to a hepatocyte.
Dependencies are inferred from the claim text.