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US 10,537,529 B2Granted
Cell-free methods of producing vault particles and vault particles resulting therefrom
AssigneeThe Regents of the University of California
InventorJan Mrazek
Life of this patent
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Abstract
Disclosed herein are methods of making vaults and packaging one or more passenger molecules in the internal cavities of the vault particles using cell-free techniques. Vaults according to the present invention and compositions comprising the vaults are free of cellular debris.
Claims16
A cell-free method of making a vault structure by polyribosome templating, which comprises
- incubating a synthesis mixture comprising a polyribosome having an mRNA encoding a major vault protein to produce multiple copies of the major vault protein that are templated into the vault structure.
A cell-free method of making a vault structure by polyribosome templating, which comprises
- adding a nucleic acid molecule that encodes a major vault protein to a synthesis mixture comprising ribosomes, and
- incubating the synthesis mixture comprising the nucleic acid molecule for 24 hours or more whereby a polyribosome forms and templates copies of the major vault protein into the vault structure.
Dependencies are inferred from the claim text.