Stabilization of glutamate dehydrogenase in an aqueous solution
AssigneeBiomerieux
InventorsFlorence Bettsworth, Jérôme Martinez
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Abstract
A process for stabilizing glutamate dehydrogenase (GDH) from a bacterium of the Clostridium genus, in an aqueous solution, in order to maintain the antigenic properties thereof, includes the step of mixing the glutamate dehydrogenase and a stabilizing composition which is a carboxylic acid having a carbon-based chain of at least 3 carbon atoms and comprising at least 2 —COOH groups, or a salt thereof. GDH compositions thus stabilized and a method of detecting the presence of bacteria of the Clostridium genus are also disclosed.
Claims34
A process for stabilizing glutamate dehydrogenase from a bacterium of the Clostridium genus in order to maintain antigenic properties of the glutamate dehydrogenase, comprising:
- stabilizing the glutamate dehydrogenase in an aqueous solution comprising (i) the glutamate dehydrogenase in a concentration ranging from 0.75 to 10 ng/ml, (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof, (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase, and (iv) a buffer;
- and
- maintaining the antigenic properties of the glutamate dehydrogenase during storage of the aqueous solution, wherein:
- the aqueous solution has a pH of between 4.5 and 7;
- and
- the aqueous solution comprises 20 to 100 molecules of the stabilizing compound per glutamate dehydrogenase monomer.
An aqueous composition comprising:
- (i) glutamate dehydrogenase from a bacterium of the Clostridium genus in a concentration ranging from 0.75 to 10 ng/ml;
- (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof;
- (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase;
- and
- (iv) a buffer, wherein:
- the aqueous composition has a pH of between 4.5 and 7;
- and
- the aqueous composition comprises 20 to 100 molecules of the stabilizing compound per glutamate dehydrogenase monomer.
A process for stabilizing glutamate dehydrogenase from a bacterium of the Clostridium genus in order to maintain antigenic properties of the glutamate dehydrogenase, comprising:
- stabilizing the glutamate dehydrogenase in an aqueous solution comprising (i) the glutamate dehydrogenase in a concentration ranging from 0.75 to 10 ng/ml, (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof, and (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase;
- and
- maintaining the antigenic properties of the glutamate dehydrogenase during storage of the aqueous solution, wherein:
- the aqueous solution has a pH of between 4.5 and 7;
- and
- the aqueous solution comprises 108 to 1010 molecules of the stabilizing compound per glutamate dehydrogenase monomer.
An aqueous composition comprising:
- (i) glutamate dehydrogenase from a bacterium of the Clostridium genus in a concentration ranging from 0.75 to 10 ng/ml;
- (ii) a stabilizing compound that is a carboxylic acid having a carbon-based chain of at least three carbon atoms and comprising at least two —COOH groups, or a salt thereof;
- and
- (iii) any of a monosaccharide polyol, disaccharide polyol, or polymeric macromolecule in addition to the glutamate dehydrogenase;
- and
- the aqueous composition has a pH of between 4.5 and 7;
- and
- the aqueous composition comprises 108 to 1010 molecules of the stabilizing compound per glutamate dehydrogenase monomer.
Dependencies are inferred from the claim text.