Method for the production of fine chemicals using a Corynebacterium secreting modified α-1,6-glucosidases

An isolated polynucleotide encoding a fusion polypeptide comprising the amino acid sequences a), b) and c) wherein:

• a) is an N-terminal Tat-signal peptide comprising the amino acid sequence of positions 1 to 33 of SEQ ID NO:10 or positions 1 to 33 of SEQ ID NO:12 or
• comprising the amino acid sequence of positions 1 to 33 of SEQ ID NO:10 with Ala at position 13 or positions 1 to 33 of SEQ ID NO:12 with Ala at position 13;
• b) is a C-terminal polypeptide having α-1,6-glucosidase activity comprising an amino acid sequence selected from the group consisting of:
• b1) a sequence ≥95% identical to the sequence from positions 37 to 639 of SEQ ID NO:10;
• and
• b2) at least (≥) 95% identical to the sequence from positions 37 to 643 of SEQ ID NO:12, and
• c) is 0 to 10 amino acid residues between a) and b).