[S,S]-EDDS biosynthesis genes and proteins and method of biosynthesis of [S,S]-EDDS
OwnerEfthimia Stegmann, Wolfgang Wohlleben +2inventors
InventorsEfthimia Stegmann, Wolfgang Wohlleben, Marius Spohn, Tilmann Weber
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Abstract
An isolated protein or peptide, which is functional for a partial synthesis step of the biosynthesis of [S,S]-ethylenediamine-disuccinate, including or composed of an amino acid sequence selected from the group consisting of SEQ ID No. 39, SEQ ID No. 41, SEQ ID No. 43, SEQ ID No. 45, SEQ ID No. 47, SEQ ID No. 49, SEQ ID No. 51, SEQ ID No. 53, and combinations thereof.
Claims4
An expression vector, including 1) at least one nucleic acid including or composed of a nucleic acid sequence selected from the group consisting of
- a) a nucleic acid sequence encoding for a protein or peptide which is functional for a partial synthesis step of the biosynthesis of [S,S]-ethylenediamine-disuccinate, including or composed of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 39, SEQ ID NO:
- 41, SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 53, and combinations thereof;
- b) a nucleic acid sequence differing from the nucleic acid sequence according to a) in the exchange of at least one codon for a synonymous codon;
- c) a nucleic acid sequence corresponding to the complementary strand of the nucleic acid sequence according to a), and combinations thereof, or
- a gene cluster or operon including or composed of at least two nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 40, SEQ ID NO:
- 42, SEQ ID NO:
- 44, SEQ ID NO:
- 46, SEQ ID NO:
- 48, SEQ ID NO:
- 50, SEQ ID NO:
- 52, SEQ ID NO:
- 54, and combinations thereof;
- and
- 2) a promoter that is not subject to zinc regulation.
A host cell, including at least one protein or peptide which is functional for a partial synthesis step of the biosynthesis of [S,S]-ethylenediamine-disuccinate, including or composed of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 39, SEQ ID NO:
- 41, SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 53, and combinations thereof, at least one nucleic acid including or composed of a nucleic acid sequence selected from the group consisting of
- a) a nucleic acid sequence encoding for a protein or peptide, which is functional for a partial synthesis step of the biosynthesis of [S,S]-ethylenediamine-disuccinate, including or composed of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 39, SEQ ID NO:
- 41, SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 53, and combinations thereof;
- b) a nucleic acid sequence differing from the nucleic acid sequence according to a) in the exchange of at least one codon for a synonymous codon;
- and
- c) a nucleic acid sequence corresponding to the complementary strand of the nucleic acid sequence according to a), and combinations thereof;
- a gene cluster or operon including or composed of at least two nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 40, SEQ ID NO:
- 42, SEQ ID NO:
- 44, SEQ ID NO:
- 46, SEQ ID NO:
- 48, SEQ ID NO:
- 50, SEQ ID NO:
- 52, SEQ ID NO:
- 54, and combinations thereof, and/or
- an expression vector, including at least one nucleic acid including or composed of a nucleic acid sequence selected from the group consisting of
- a) a nucleic acid sequence encoding for a protein or peptide, which is functional for a partial synthesis step of the biosynthesis of [S,S]-ethylenediamine-disuccinate, including or composed of an amino acid sequence selected from the group consisting of SEQ ID NO:
- 39, SEQ ID NO:
- 41, SEQ ID NO:
- 43, SEQ ID NO:
- 45, SEQ ID NO:
- 47, SEQ ID NO:
- 49, SEQ ID NO:
- 51, SEQ ID NO:
- 53, and combinations thereof;
- b) a nucleic acid sequence differing from the nucleic acid sequence according to a) in the exchange of at least one codon for a synonymous codon;
- and
- c) a nucleic acid sequence corresponding to the complementary strand of the nucleic acid sequence according to a), and combinations thereof;
- and
- a gene cluster or operon, including or composed of at least two nucleic acid sequences selected from the group consisting of SEQ ID NO:
- 40, SEQ ID NO:
- 42, SEQ ID NO:
- 44, SEQ ID NO:
- 46, SEQ ID NO:
- 48, SEQ ID NO:
- 50, SEQ ID NO:
- 52, SEQ ID NO:
- 54, and combinations thereof, wherein zinc repression is not allowed in the host cell.
Dependencies are inferred from the claim text.