Modified virus-like particles of CMV
AssigneeSaiba GmbH
InventorsMartin Bachmann, Andris Zeltins, Paul Pumpens
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Abstract
The present invention relates to virus-like particles of plant virus Cucumber Mosaic Virus (CMV), and in particular to modified VLPs of CMV comprising Th cell epitopes, in particular universal Th cell epitopes. Furthermore, these modified VLPs serve as, preferably, vaccine platform, for generating immune responses, in particular antibody responses, against antigens linked to said modified VLPs. The presence of the Th cell epitopes, in particular universal Th cell epitopes, led to a further increase in the generated immune response.
Claims22
A modified virus-like particle (VLP) of cucumber mosaic virus (CMV), wherein said modified VLP of CMV comprises at least one modified CMV polypeptide, wherein said modified CMV polypeptide comprises
- (a) a CMV polypeptide, and
- (b) a T helper cell epitope, wherein said T helper cell epitope replaces an N-terminal region of said CMV polypeptide, wherein said N-terminal region of said CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO:1;
- and
- wherein said CMV polypeptide comprises
- (i) an amino acid sequence of a coat protein of CMV;
- or
- (ii) a mutated amino acid sequence, wherein the amino acid sequence to be mutated is an amino acid sequence of a coat protein of CMV, and wherein said mutated amino acid sequence and said coat protein of CMV show a sequence identity of at least 90%.
Dependencies are inferred from the claim text.