Method for obtaining a composition for biomass hydrolysis
AssigneesInternational Centre for Genetic Engineering and Biotechnology, Department of Biotechnology Ministry of Science & Technology
InventorsSyed Shams Yazdani, Funso Emmanuel Ogunmolu
Life of this patent
Estimating the term…
Abstract
The present invention discloses a composition for biomass hydrolysis. The components of the composition may be obtained by biological or synthetic means. Synthetically, the components of the composition of the present invention may be obtained by amino acid synthesis or may be procured commercially.
Claims11
A composition for hydrolysis of biomass comprising;
- a. Cellobiohydrolase I (GH7-CBM1) in the range of 50%-75%, beta-D-glucosideglucohydrolase (GH3) in the range of 1%-4%, xylanase (GH11-CBM1) in the range of 1%-6%, swollen in in the range of 7%-13%, GMF family protein in the range of 1%-5%, IgE-binding protein in the range 1%-3%, Hydrophobic surface binding protein A in the range 1%-3%, endoglucanase (GH5-CBM1) in the range of 2%-25%, and LPMO (AA9) in the range of 1%-5%,
- b. optionally, comprising, cellobiohydrolase II (GH6-CBM1) in the range of 25%-40%, endoglucanase GH45 in the range of 0.50%-2.5%, alpha-L-arabinofuranosidase in the range of 2%-5%, acetyl xylan esterase (CE5-CBM1) in the range of (15%-30%), carbohydrate esterase (CE3-CBM1) in the range of 0.30%-2%, Glycoside Hydrolase family 18 protein (GH 18) in the range of 1%-4%, and glucoamylase (GH15-CBM20) in the range of 8%-15%,
- wherein, the composition is obtained from Penicillium funiculosum (NCIM1228).
The method of producing the composition as claimed in claimed in 2, wherein, secretome were analysed and selected on biomass hydrolysis.
Dependencies are inferred from the claim text.