Methods for detecting and quantifying non-polar analytes with high sensitivity
AssigneeWaters Technologies Corporation
InventorMichael S. Young
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Abstract
The technology of the present application is directed to methods and kits for detecting and quantifying a non-polar analyte in a plant-derived sample. The technology uses a simple extraction, e.g., a liquid-liquid extraction (LLE) or solid-phase extraction (SPE), to enrich a sample for a non-polar analyte of interest and to remove contaminants. After the extraction and clean-up steps, liquid chromatography and mass spectrometry are used to detect the non-polar analyte. In one embodiment, acequinocyl and/or its derivatives is analyzed using liquid chromatography and tandem mass spectrometry (LC-MS/MS) and the improved LC-MS/MS conditions allows detection limits of acequinocyl and/or its derivatives of 50 ppb or less.
Claims20
A method for detecting and quantifying a non-polar analyte in a plant-derived sample, comprising:
- (a) purifying said sample in a first purification step to obtain a first solution;
- (b) purifying said first solution in a second purification step comprising extracting said first solution with liquid-liquid extraction (LLE) or solid phase extraction (SPE) using a non-polar solvent to obtain a second solution;
- and
- (c) subjecting said second solution to detection and quantitative analysis;
- wherein when LLE is used, said non-polar analyte is retained in a non-polar liquid phase, and said second solution is said non-polar liquid phase comprising said non-polar analyte;
- and
- wherein when SPE is used, said non-polar analyte is initially retained on a non-polar solid phase and is then eluted from said non-polar solid phase in a separate elution step, and said second solution is said eluate from said non-polar solid phase comprising said non-polar analyte.
Dependencies are inferred from the claim text.