Method of screening for candidate compounds for regulating melanogenesis or pigmentation
AssigneesChanel Parfums Beaute, National Institute of Advanced Industrial Science and Technology
InventorsRenu Wadhwa, Sunil Kaul, Nobuhiro Ando, Christian Mahe
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Abstract
A method to identify target genes or proteins for regulating melanogenesis or pigmentation and to screen for compounds for manipulation of melanogenesis or pigmentation, the method of screening for candidate compounds for regulating melanogenesis or pigmentation, includes bringing test compounds into contact with cells capable of expressing mortalin and/or Hsp60 in vitro, and selecting, from among the test compounds, a compound that changes the expression level of mortalin and/or Hsp60.
Claims3
An in vitro method of screening for candidate cosmetic compounds for regulating melanogenesis or pigmentation, comprising the steps of:
- (i) determining by enzyme-linked immunosorbent assay (“ELISA”), in a sample of cells expressing mortalin and/or heat shock protein 60 (Hsp60), the expression level of mortalin and/or Hsp60;
- (ii) bringing a test compound into contact with said sample of cells expressing mortalin and/or Hsp60 in vitro;
- (iii) determining by ELISA, in said cells from step (ii), the expression level of mortalin and/or Hsp60;
- (iv) identifying a change in the expression level of mortalin and/or Hsp60 measured in step (iii) as compared to the expression level of mortalin and/or Hsp60 measured in step (i), and
- (v) selecting said candidate cosmetic compound for possible incorporation into a cosmetic composition for regulating melanogenesis or pigmentation.
Dependencies are inferred from the claim text.