Method for dissociating water using photosystem II (PSII)

A method for dissociating an aqueous solution, comprising:

• first electrochemical oxidating the aqueous solution in the presence of pulsed light, said pulsed light being generated from a first source of light energy at a predetermined pulse frequency value, and having a predetermined peak power, by an enzymatic composition based on a first enzymatic complex PSII, isolated from a second enzymatic complex PSI, with production of oxygen, free electrons and free protons in the aqueous solution, wherein said light energy from said first source of light energy has a variable energy value over time, said variable energy value over time having a light energy value per second determined over a plurality of pulses of said pulsed light;
• capturing said free electrons and of said free protons;
• capturing oxygen;
• and
• modulating said predetermined pulse frequency value of said pulsed light to a sufficient pulse frequency value in order to obtain a first oxygen production yield per unit of light energy which is greater by a factor (f) of 1.01 to 100.00 than a second oxygen production yield per unit of light energy obtained for a second electrochemical oxidation in the presence of continuous light,
• said first and second oxygen production yields (ηi) being each obtained from an oxygen production rate (K(O2)i) per unit of light energy, i=1 or 2, such that:
• η
• i
• =
• K
• ⁡
• (
• O
• 2
• )
• i
• E
• i
• ,
• where Ei refers to an amount of energy per second provided for obtaining said oxygen production rate (K(O2)i), i=1 or 2,
• said factor (f) being equal to a ratio between said first oxygen production yield (η1) and said second oxygen production yield (η2) such that:
• f
• =
• η
• 1
• η
• 2
• ,
• said pulsed light and said continuous light having an equal predetermined wavelength and said continuous light have an equal power to said predetermined peak power of said pulsed light.