Phage insensitive streptococcus thermophilus
AssigneeDsm IP Assets B.V.
InventorsRoelof Hendrik Matthijs Kouwen, Pim Van Hee, Douwe Van Sinderen, Brian McDonnell, Jennifer Mahony
Life of this patent
Estimating the term…
Abstract
Bacteriophage Insensitive Mutants (BIMs) of three Streptococcus thermophilus parent strains were generated and characterized for phage sensitivity, sedimentation rate, cell chain length, phage adsorption and CRISPR loci alterations. Several BIMs showed an altered sedimentation phenotype as well as an increase cell chain length, reduced phage sensitivity, reduced phage adsorption and 100% identity in three CRISPR loci. The results show that the derived BIMs have become phage-resistant through a mechanism other than CRISPR.
Claims20
A bacteriophage insensitive mutant of a parent strain of Streptococcus thermophilus, wherein the bacteriophage insensitive mutant is generated by exposing the Streptococcus thermophilus parent strain to an isolated bacteriophage wherein the bacteriophage insensitive mutant is suitable for use in food or feed, and wherein the bacteriophage insensitive mutant has an increased sedimentation rate and/or an increased chain formation compared to the parent strain, wherein the parent strain is deposited as CBS136255, CBS136256 or CBS138555.
Dependencies are inferred from the claim text.