Bacteriophage detection
AssigneeDsm IP Assets B.V.
InventorsRoelof Hendrik Matthijs Kouwen, Douwe Van Sinderen, Brian McDonnell
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Abstract
The present invention relates to a kit suitable for detection of Streptococcus thermophilus infecting phages, said kit comprising: (a) a first primer pair suitable to generate an amplicon characteristic for a 987 phage; and (b) a second primer pair suitable to generate an amplicon characteristic for a 5093 phage. Further, the present invention relates to a method for detecting the presence of Streptococcus thermophilus infecting phages in a sample.
Claims8
A method for detecting the presence of Streptococcus thermophilus infecting phages in a sample, comprising
- (i) providing a sample;
- and
- (ii) performing a multiplex PCR analysis of the sample using a first primer pair suitable to generate an amplicon from nucleic acid sequences in a 987 phage and a second primer pair suitable to generate an amplicon from nucleic acid sequences in a 5093 phage,
- wherein the first primer pair comprises the forward primer comprising the nucleotide sequence of SEQ ID NO:
- 1 and the reverse primer comprising the nucleotide sequence of SEQ ID NO:
- 2, and wherein said 987 phage amplicon is 707 base pairs in length;
- and wherein the second primer pair comprises the forward primer comprising the nucleotide sequence of in SEQ ID NO:
- 3 and the reverse primer comprising the nucleotide sequence of SEQ ID NO:
- 4, and wherein said 5093 phage amplicon is 983 base pairs in length.
A method for detecting the presence of Streptococcus thermophilus infecting phages in a sample, comprising
- (i) providing a sample;
- and
- (ii) performing a multiplex PCR analysis of the sample using a first primer pair suitable to generate an amplicon from nucleic acid sequences in a 987 phage and a second primer pair suitable to generate an amplicon from nucleic acid sequences in a 5093 phage, wherein said first primer pair is specific for the nucleotide sequence of the conserved scaffolding protein-encoding ORF69871 gene and wherein said second primer pair is specific for the nucleotide sequence of the portal protein-encoding ORF30095 gene.
Dependencies are inferred from the claim text.