US 10,519,500 B2 · Crystalline copper-based coordination polymers and their use

Abstract

A method of preparing a crystalline copper-based coordination polymer comprises preparing a mixture of copper ions and a first quaternized carboxylate pyridyl ligand; adding a second polypyridyl ligand; and forming crystals of a copper-based coordination polymer therefrom. The crystalline copper-based coordination polymer is suitable for providing a sensing platform for detecting the presence of one or more target nucleic acid sequences such as viral RNA, in particular Dengue virus and/or Zika virus RNA with high selectivity and high specificity. A method of detecting at least a first target nucleic acid sequence, in particular from a viral RNA, in particular it is Flavivirus RNA, in a sample is also provided. Further provided is a kit including the crystalline copper-based coordination polymer and an oligonucleotide probe.

Claims17

Dependent claims

A method of preparing a crystalline copper-based coordination polymer, that method comprises steps of:

(i) preparing a mixture comprising copper ions and a first pyridyl ligand which first pyridyl ligand is a quaternized carboxylate pyridyl ligand having a structure of Formula (IV):
with X being a halogen:
(ii) adding a second pyridyl ligand, which second pyridyl ligand is a polypyridyl ligand having a structure of Formula (V):
with L being a linking group selected from —(CH2)n′—, —(CH═CH)n′— or —(N═N)n′— and with n′ being an integer and selected from 0, 1, 2 or 3;
(iii) subjecting the mixture of step (ii) to conditions under which crystals of the copper-based coordination polymer are formed;
(iv) separating the crystals of the copper-based coordination polymer from the mixture.

3 more elements

Dependencies are inferred from the claim text.

Crystalline copper-based coordination polymers and their use

Life of this patent

Crystalline copper-based coordination polymers and their use

Life of this patent

Abstract

Claims17