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US 10,519,498 B2Granted
Additives to improve sequencing by synthesis performance
AssigneeQiagen Sciences, LLC
InventorJames J. DiMeo
Life of this patent
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Abstract
The invention relates to methods, compositions, devices, systems and kits are described including, without limitation, reagents and mixtures, for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods.
Claims9
A method of incorporating nucleotides, comprising:
- a) providing i) a plurality of nucleic acid primers and template molecules, ii) an extend reagent comprising polymerase and a plurality of nucleotide analogues, and iii) a wash reagent comprising methylenediphosphonic acid;
- b) hybridizing at least a portion of said primers to at least a portion of said template molecules so as to create hybridized primers;
- c) exposing said hybridized primers to said extend reagent under conditions such that a first nucleotide analogue is incorporated into at least a portion of said hybridized primers so as to create extended primers comprising an incorporated nucleotide analogue;
- d) washing said extended primers with said wash reagent.
1 more element
Dependencies are inferred from the claim text.