Integrated device for nucleic acid detection and identification

A method for detecting a target nucleic acid, the method consisting of:

• disposing a sample comprising the target nucleic acid in a sample chamber of a disposable platform;
• orienting the disposable platform vertically or at a tilt;
• reacting the sample with a liquid or previously lyophilized amplification reagent mix;
• opening a first vent pocket connected via a first vent channel to an amplification chamber to lower pressure, thereby enabling the reacted sample to flow into the amplification chamber;
• amplifying the target nucleic acid in the amplification chamber;
• opening a second vent pocket connected via a second vent channel to a labeling chamber to lower pressure, thereby enabling the amplified target nucleic acid to flow into the labeling chamber;
• labeling the amplified target nucleic acid using detection particles in the labeling chamber;
• opening a third vent pocket connected via a third vent channel to a detection subsystem to lower pressure, thereby enabling the labeled target nucleic acid to flow into the detection subsystem;
• and
• detecting the amplified target nucleic acid;
• wherein said vent pockets are separate from said amplification chamber, said labeling chamber, and said detection subsystem such that said vent channels remain clear of fluid;
• and
• wherein each opening step comprises rupturing a heat labile material sealing each vent pocket from lower pressure.