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US 10,519,485 B2Granted
Dual quencher probes
AssigneeBiosearch Technologies, Inc.
InventorsRonald M. Cook, Ben Ayer, Marc P. Beal
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Abstract
The present invention provides nucleic acid probes comprising two quenchers of excited state energy, and methods of their use.
Claims56
A nucleic acid probe comprising:
- an oligonucleotide having the structure 5′-Y1-L1-LQ1-L2-Y2-3′, wherein
- Y1 comprises a sequence of two or more DNA or RNA nucleotides;
- Y2 comprises a sequence of two or more DNA or RNA nucleotides;
- wherein one of Y1 and Y2 has a fluorophore covalently attached to its nucleotide sequence, and the other of Y1 and Y2 has a second quencher covalently attached to its nucleotide sequence;
- L1 and L2 are independently selected from a bond, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heteroalkyl, and substituted or unsubstituted heterocycloalkyl;
- and
- LQ1 is:
- wherein Rb2 and Rc2 are independently selected alkyl moieties.
5 more elements
Dependencies are inferred from the claim text.