US 10,519,484 B2 · Lytic composition and application thereof, kit, method for preparing nucleic acid by utilizing lytic composition, and nucleic acid analysis method

Abstract

The present invention relates to the technical field of biology, and in particular relates to a lytic composition and an application thereof, a kit, a method for preparing nucleic acid by utilizing the lytic composition, and a nucleic acid analysis method. A lytic product obtained in the present invention can be directly used for the nucleic acid analysis method without purifying the nucleic acid. According to the lytic composition disclosed by the present invention, multiple biological tissues and cells are lysed so as to release the nucleic acid of the cells into a solution, the nucleic acid in a whole-cell lysing and mixing state directly serves as a template without using a conventional nucleic acid separation and purification process after termination of the reaction, and nucleic acid amplification analysis is guided. For example, nucleic acid amplification is performed by a real-time fluorescence qPCR, etc.

Claims11

Dependent claims

A lytic composition, wherein the composition is formed by combining A component and B component, A is Ca(OH)2, and B is PEG with a molecular weight of 100;

the concentration of A is 0.1 mmol/L, and the concentration of B is 200 mg/mL;
a molar ratio of A to B is 0.1:1.05;
and the lytic composition further comprises a reaction termination product.

Dependencies are inferred from the claim text.

Facts

Application

15/472,510Filed 29 Mar 2017 · continuation

Priority

CN 2014 1 052282130 Sep 2014

Published

US 2017/0283856 A15 Oct 2017

Granted

31 Dec 2019

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12Q 1/6806
Involving nucleic acids: preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12N 15/10
Recombinant DNA-technology: processes for the isolation, preparation or purification of DNA or RNA
C12N 15/1003
Processes for the isolation, preparation or purification of DNA or RNA: extracting or separating nucleic acids from biological samples, e.g. pure…
G01N 1/28
Sampling; Preparing specimens for investigation: preparing specimens for investigation including physical details of chemical methods covered…

Lytic composition and application thereof, kit, method for preparing nucleic acid by utilizing lytic composition, and nucleic acid analysis method

Life of this patent

Lytic composition and application thereof, kit, method for preparing nucleic acid by utilizing lytic composition, and nucleic acid analysis method

Life of this patent

Abstract

Claims11