Method of detecting a Salmonella microorganism

A method of detecting a microorganism, the method comprising:

• providing,
• a sample to be tested;
• a culture device;
• a nutrient medium that facilitates growth of a Gram-negative enteric microorganism;
• a first selective agent that inhibits the growth of Gram-positive microorganisms;
• a first differential indicator system comprising a first differential indicator compound that can be converted to a first detectable product by a member of a group of Salmonella microorganisms that includes a microorganism of the species Salmonella bongori;
• a second differential indicator system comprising a second differential indicator compound that can be converted to a second detectable product by a β-galactosidase enzyme activity;
• and
• a third differential indicator system comprising a third differential indicator compound that can be converted by a Salmonella microorganism to a third detectable product;
• contacting in the culture device the nutrient medium, the first selective agent, the first differential indicator system, and the second differential indicator system with a sample to form an inoculated culture device;
• incubating the inoculated culture device for a first period of time at a temperature greater than 40 degrees C.;
• detecting the presence of the first detectable product;
• detecting the absence of the second detectable product;
• and
• detecting the presence or absence third detectable product;
• wherein observing the presence of the third detectable product indicates the presence of a Salmonella microorganism in the sample;
• and
• wherein observing the absence of the third detectable product indicates the absence of a Salmonella microorganism in the sample.