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US 10,519,478 B2Granted
Method of producing substance
AssigneeUbe Industries, Ltd.
InventorsMasahiko Hagihara, Motohisa Shimizu, Yukinori Wada
Life of this patent
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Abstract
The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.
Claims15
A method of producing a substance using cells, the method including the steps of:
- applying the cells to a porous polyimide film,
- culturing the cells in the porous polyimide film to produce the substance by the cells, and
- recovering the substance,
- wherein the porous polyimide film has a three-layer structure consisting of an A-surface layer having a plurality of pores, a B-surface layer having a plurality of pores, and a macro-void layer sandwiched between the two surface layers,
- a mean pore size in the A-surface layer is smaller than a mean pore size in the B-surface layer, and
- the macro-void layer has a partition bonded to the A-surface layer and the B-surface layer, and a plurality of macro-voids surrounded by the partition, the A-surface layer, and the B-surface layer,
- wherein the substance is selected from the group consisting of proteins, glycoproteins and viruses.
4 more elements
A method of producing a substance using cells, the method including the steps of:
- applying the cells to a porous polyimide film,
- culturing the cells in the porous polyimide film to produce the substance by the cells, and
- recovering the substance,
- wherein the porous polyimide film has a three-layer structure consisting of an A-surface layer having a plurality of pores, a B-surface layer having a plurality of pores, and a macro-void layer sandwiched between the two surface layers,
- a mean pore size in the A-surface layer is smaller than a mean pore size in the B-surface layer, and
- the macro-void layer has a partition bonded to the A-surface layer and the B-surface layer, and a plurality of macro-voids surrounded by the partition, the A-surface layer, and the B-surface layer,
- wherein the cells are cultured under static culture conditions, or the cells are cultured under rotating culture or stirred conditions.
4 more elements
Dependencies are inferred from the claim text.