Recombinant microorganism for improved production of fine chemicals

A recombinant microorganism having a higher yield and/or productivity of alanine in fermentative production compared to a respective reference microorganism, the recombinant microorganism comprising:

• (A) a reduced, repressed or deleted activity and/or expression of a dadX gene, wherein the dadX gene is selected from the group consisting of:
• (i) a nucleic acid molecule comprising SEQ ID NO:
• 118,
• (ii) a nucleic acid molecule having at least 80% identity to SEQ ID NO:
• 118,
• (iii) a nucleic acid molecule encoding the polypeptide comprising SEQ ID NO:
• 119, and
• (iv) a nucleic acid molecule encoding a polypeptide having at least 80% identity to SEQ ID NO:
• 119,
• and
• (B) at least one of:
• a) a reduced, repressed or deleted activity and/or expression of a brnQ gene or a homolog or functional variant thereof,
• b) an introduced, increased, enhanced or altered activity and/or expression of a argP gene or a homolog or functional variant thereof, wherein the argP gene or a homolog or functional variant thereof is selected from:
• (I) a nucleic acid molecule comprising SEQ ID NO:
• 45 or a nucleic acid molecule having at least 80% identity to SEQ ID NO:
• 45, wherein the codon encoding the amino acid alanine corresponding to position 286 to 288 of SEQ ID NO:
• 45 has been replaced by a codon encoding the amino acid glutamic acid or an amino acid related to glutamic acid, or
• (II) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:
• 46 or a polypeptide having 80% identity to SEQ ID NO:
• 46, wherein the amino acid of the protein corresponding to position 96 of SEQ ID NO:
• 46 is not alanine,
• c) an introduced, increased, enhanced or altered activity and/or expression of a gcvA gene or a homolog or functional variant thereof, and/or
• d) an altered lpxD gene or a functional homologous gene thereof, wherein the lpxD gene or functional homologous gene thereof is selected from:
• (I) a nucleic acid molecule comprising SEQ ID NO:
• 49 or a nucleic acid molecule having at least 80% identity to SEQ ID NO:
• 49, wherein the codon corresponding to position 43 to 45 of SEQ ID NO:
• 49 is encoding amino acid threonine or an amino acid related to threonine, or
• (II) a nucleic acid molecule encoding the polypeptide of SEQ ID NO:
• 50 or a polypeptide having at least 80% identity to SEQ ID NO:
• 50, wherein the amino acid of the protein corresponding to position 15 of SEQ ID NO:
• 50 is not alanine.