Recombinant host cell for biosynthetic production
AssigneeRhodia Operations
InventorsOdile Ramaen, Vincent Sauveplane, Rudy Pandjaitan
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Abstract
A cell may include heterologous polynucleotides encoding a multienzyme complex involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, which multienzyme complex comprises enzymes for the biosynthesis of coumaric acid and a crotonase.
Claims23
A yeast cell comprising heterologous polynucleotides encoding a multienzyme complex, wherein the multienzyme complex is involved in the metabolic pathway of phenylpropanoids and biosynthesis of a vanilloid or a hydroxybenzaldehyde precursor thereof, and wherein the multienzyme complex comprises:
- a) a crotonase, b) a CoA ligase, c) a 3-monooxygenase;
- and d) a methyltransferase;
- wherein a) is enoyl-CoA hydratase (ECH), b) is 4-coumarate-CoA ligase (4CL), c) is hydroxybenzoic acid hydroxylase (HBH), or phenolhydroxylase (PheA) and flavinreductase (FLARED);
- and d) is O-methyltransferase.
Dependencies are inferred from the claim text.