Wheat MS1 polynucleotides, polypeptides, and methods of use

A method for propagating male-sterile wheat plants homozygous for male sterile 1 (ms1), comprising

• (a) pollinating the male-sterile wheat plants with a maintainer wheat plant homozygous for ms1, wherein said maintainer wheat plant is hemizygous for a DNA construct which comprises:
• (i) a polynucleotide encoding an amino acid sequence that is at least 95% identical to the full length of the amino acid sequence of SEQ ID NO:5, wherein the polynucleotide when expressed in the msl male-sterile wheat plants restores male fertility to the ms1 male-sterile wheat plants;
• (ii) a polynucleotide encoding a gene product which inhibits the formation, function, or dispersal of male gametes of the maintainer plant;
• and
• (iii) a polynucleotide encoding a screenable marker for seed selection;
• (b) harvesting seed produced by the male-sterile wheat plants;
• (c) selecting seeds that do not comprise the marker for seed selection;
• and
• (d) planting the selected seed to grow male-sterile wheat plants
• wherein each of the polynucleotides listed in (i), (ii) and (iii) are operably linked to a promoter, wherein the promoter for the polynucleotide of (i) and (ii) is a male-fertility promoter and wherein the promoter for the polynucleotide of (iii) is a seed-preferred promoter.