Soybean U6 polymerase III promoter and methods of use
AssigneeE. I. Du Pont de Nemours and Company
InventorsZhongsen Li, Zhan-Bin Liu
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Abstract
The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.
Claims18
A recombinant DNA construct comprising a U6 polymerase III promoter nucleotide sequence having at least 98% sequence identity to the full length of SEQ ID NO:
- 120, operably linked to at least one heterologous sequence, wherein said nucleotide sequence controls the expression of a heterologous sequence.
A plant stably transformed with a recombinant DNA construct comprising a soybean promoter and a heterologous nucleic acid fragment operably linked to said promoter, wherein said promoter controls expression of said heterologous nucleic acid fragment in a plant cell, and further wherein said promoter comprises a nucleotide sequence having at least 98% sequence identity to the full length of SEQ ID NO:
- 120.
A recombinant DNA construct comprising a soybean U6 polymerase III promoter comprising a nucleotide sequence having at least 98% sequence identity to the full length of SEQ ID NO:120, wherein said promoter is operably linked to a heterologous nucleic acid fragment, and wherein said heterologous nucleic acid fragment expresses a guide RNA.
Dependencies are inferred from the claim text.